Description:The easiest and most convenient way to run PCR: squeeze then run. User can squeeze one drop of PCR Master mix into PCR tube/plate, add template and primers, and then can run PCR reaction. This PCR Master Mix contains Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. This unique Mix recipe makes the system very reliable, and tolerant to most of the PCR condition (concentration of the components).The Taq Polymerase in the PCR Master Mix is Hot Start polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. So no need to prepare the reactions on ice. The Polymerase is activated by a 10 minutes, 95°C incubation.This PCR Master Mix is very stable, and can be stored in 4oC for up to 6 months. No need to wait the thawing.The amplification range of the Taq is ~ 6 kb.
The PCR Master Mix contains dye, and can directly run electrophoresis after PCR reaction.